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Image Search Results
Journal: bioRxiv
Article Title: Efficacy of Targeting SARS-CoV-2 by CAR-NK Cells
doi: 10.1101/2020.08.11.247320
Figure Lengend Snippet: ( a ) Immunophenotyping of CR3022-CAR. Antibodies against various immunomodulatory receptors including TIGIT, LAG-3, TIM-3, KLRG1, CTLA-4, PD-1, CD69, CD8A, NKG2C, CD94, DNAM-1, 2B4, NKG2D, NKP46, and CD16 were used to stain CR3022-CAR and NK-92MI. The expression of these receptors was determined by flow cytometry. ( b ) Diagram of CR3022-CAR binding to the RBD domain of SARS-CoV-2-S recombinant protein. CR3022-CAR binds to RBD of SARS-CoV-2-S protein which is then recognized by anti-His and its corresponding secondary antibody conjugated to a fluorophore. ( c ) Representative dot plots of CR3022-CAR binding to RBD of SARS-CoV-2. CR3022-CAR or NK-92MI cells were incubated with SARS-CoV-2-RBD or SARS-CoV-1-RBD recombinant protein. The binding efficiency was determined by flow cytometry.
Article Snippet:
Techniques: Staining, Expressing, Flow Cytometry, Binding Assay, Recombinant, Incubation